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Tranche to Mascot
An example tool exists that shows how Tranche data can be directly sent to a Mascot (Matrix Science, Ltd) server. Normally Mascot users must copy data to their local hard drive, then upload the data via a web browser to Mascot. The Tranche to Mascot bridge is an example of how Tranche may be used to store data in a fashion where the user need not ever have a copy of the peak lists they are searching on Mascot.
The entire tool looks just like the normal use of Mascot. Additionally, the Tranche to Mascot bridge will work on any on-line instance of Mascot. By default the public Mascot server is used. Data may be sent to Mascot by using the following link.
http://www.proteomecommons.org/data/mascotsearch.html?uri={Tranche Hash}
Where {Tranche Hash} is replaced with the Tranche hash of the peak list file to search. For example, this link would search a peak list from the Aurum data set.
The nice thing about the Tranche to Mascot bridge is that the code is dynamically taking the HTML from the public Mascot server. If Matrix Science updates Mascot or a custom version of Mascot is used, the Tranche to Mascot bridge should remain unaffected.
Links of interest
Screenshots
- Step #1: Fill out search params (note you don't pick a file)
- Step #2: Send the search to Mascot
- Step #3: View results directly from Mascot
Credits/References
The Tranche to Mascot bridge code was designed and implemented by Jayson Falkner and Phil Andrews. The source-code for the bridge may be found with the ProteomeCommons.org Tranche source-code.