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Sequest
Sequest correlates tandem mass spectra of peptides with amino acid databases. For more information, please see the Sequest website.
If you are citing the SQT-file generator in Tranche, please use this citation information.
- Link to this page: http://tranche.proteomecommons.org/examples/sqt/index.html
- Citation for Tranche: http://tranche.proteomecommons.org
- Citation for Sequest: http://fields.scripps.edu/sequest/
- Reference for SQT Format: http://fields.scripps.edu/sequest/SQTFormat.html
SQT File Format
To use this feature, you will need to install the module. Open up the Module Manager (from "Advanced Tools"), and select to install SQT File Generator from the server.
Sequest outputs matches in the form of out files (*.out). For larger data sets, the number of out files can be quite large. Many out files are often converted to a single SQT file for convenience.
If one or more Sequest out files are selected, you can elect to download and have Tranche convert them to a SQT file. Tranche can also convert archives of out files to SQT files.
Example
- Step 1: Find a project that contains Sequest output files. (For this example, I'm using "ServerInfoMonitoringThread". If running Tranche, wait for projects to load and filter to find the project quickly.) Open the project to browse the files.
- Step 2: Select either one or more archives or out files (*.out). Right-click (control-click) and select "Generate SQT" For this example, select "HFH_s100b_o1.tgz". [View screenshot]
- Step 3: A new frame will open with the title "Generate SQT File". Select the output file and click "Start". [View screenshot]
- Step 4: When the converter is done, a popup message will alert you concerning the location of the SQT file. [View screenshot]
- Step 5: You can open the file in a text editor to see the result of the SQT generator. [View screenshot]
The tool is quite young and needs feedback for improvement. If you have feedback, please contact us.
Other helpful information